1293 .
SYMBIOBACTERIUM THERMOPHILUM MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
7.5 - 7.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 5.0 | g | ||
|
|
NaNO3 | 1.7 | g | ||
|
|
Yeast extract
(OXOID) |
5.0 | g | ||
|
|
Tryptone
(BD Bacto) |
10.0 | g | ||
|
|
NH4HCO3 | 3.2 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients (except bicarbonate) and adjust pH to 8.0, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add bicarbonate from a sterile anoxic 3.2% (w/v) stock solution prepared under 80% N2 and 20% CO2 gas mixture (e.g., add 1 ml stock solution per 9 ml medium). Adjust pH of the complete medium to 7.5 - 7.8, if necessary. | |||||
| 2 After inoculation add sterile 100% H2 gas to an overpressure of 0.5 bar. | |||||