# 1293: SYMBIOBACTERIUM THERMOPHILUM MEDIUM


## Main sol. 1293

5	g	NaCl	
1.7	g	NaNO3	
5	g	Yeast extract (OXOID)	
10	g	Tryptone (BD Bacto)	
3.2	g	NH4HCO3	
1000	ml	Distilled water	

1. Dissolve ingredients (except bicarbonate) and adjust pH to 8.0, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add bicarbonate from a sterile anoxic 3.2% (w/v) stock solution prepared under 80% N2 and 20% CO2 gas mixture (e.g., add 1 ml stock solution per 9 ml medium). Adjust pH of the complete medium to 7.5 - 7.8, if necessary.
2. After inoculation add sterile 100% H2 gas to an overpressure of 0.5 bar.