1201
1201 .
ALICYCLOBACILLUS FERROOXYDANS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
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Medium for Alicyclobacillus ferrooxydans DSM 22381
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 1201 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 930 | ml | - | - | |
| Solution B | 70 | ml | - | - | |
| 1 Autoclave solution A, cool down to 50°C and combine solution A with 70 ml of solution B. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.50 | g | 0.538 | 2.181 | |
| (NH4)2SO4 | 0.40 | g | 0.43 | 3.255 | |
| K2HPO4 | 0.20 | g | 0.215 | 1.235 | |
| K2S4O6 | 0.15 | g | 0.161 | - | |
| KCl | 0.10 | g | 0.108 | 1.442 | |
| MnSO4 x H2O | 0.01 | g | 0.011 | 0.064 | |
| Yeast extract | 0.20 | g | 0.215 | - | |
| Distilled water | 930.00 | ml | - | - | |
| 1 Adjust pH to 1.8 - 2.5 with H2SO4. | |||||
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeSO4 x 7 H2O | 20 | g | 200 | 719.386 | |
| 1 Dissolve in 0.2 N H2SO4 and fill up to a volume of 100 ml. Filter sterilize. | |||||
Metadata
| Last modified: | 27.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Alicyclobacillus ferrooxydans DSM 22381
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| FeSO4 x 7 H2O | 14 | 50.357 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| (NH4)2SO4 | 0.4 | 3.027 |
| K2HPO4 | 0.2 | 1.148 |
| Yeast extract | 0.2 | - |
| Show more | ||
