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DSMZ

DSMZ 1187 . ANOYXNATRONUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1187 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 25.0 g 25 235.876
NaHCO3 25.0 g 25 297.594
KCl 0.2 g 0.2 2.683
MgCl2 x 6 H2O 0.1 g 0.1 0.492
NH4Cl 0.5 g 0.5 9.348
K2HPO4 0.2 g 0.2 1.148
Yeast extract 0.2 g 0.2 -
Trace element solution SL-10 1.0 ml - -
Wolin's vitamin solution 1.0 ml - -
Na2S x 9 H2O 0.7 g 0.7 2.914
Glucose 5.0 g 5 27.753
Distilled water 1000.0 ml - -
1 pH 9.0
2 Prepare the medium anaerobically without the vitamins, Na2S, yeast extract NaHCO3, and Na2CO3. Boil the medium, cool under N2, add the NaHCO3, and Na2CO3, dispense under N2 and autoclave the medium. Add the vitamin solution, yeast extract, Na2S and glucose from sterile stock solution prepared under N2 to the cooled, autoclaved medium.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 25 297.594
Na2CO3 25 235.876
Glucose 5 27.753
Na2S x 9 H2O 0.7 2.914
NH4Cl 0.5 9.348
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain
oxygen obligate anaerobe 1 strain