1182 .
AQUISALIMONAS MEDIUM
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Equipment needed:
Autoclave
Final pH:
9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 500 | ml | ||
|
|
Solution B | 500 | ml | ||
| 1 Autoclave solutions A and B separately and mix when cool. The medium may be solidified by adding 20 g/l agar to solution A. Solutions A and B are then cooled to 50°C, mixed and plates poured immediately. | |||||
| 2 Final pH of the medium is 9.5. | |||||
Solution A #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Glucose | 10.0 | g |
|
|
Peptone | 5.0 | g |
|
|
Yeast extract | 5.0 | g |
|
|
K2HPO4 | 1.0 | g |
|
|
MgSO4 x 7 H2O | 0.2 | g |
|
|
Distilled water | 500.0 | ml |
|
|
Agar , for solid medium | 10.0 | g |