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DSMZ

DSMZ 1176 . HALOVIBRIO MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Halovibrio variabilis DSM 3050

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days aerobic 28 °C

Main sol. 1176 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 120.00 g 120 2053.39
K2HPO4 3.00 g 3 17.224
NH4Cl 0.50 g 0.5 9.348
Trace elements solution (Pfennig, 1965) 1.00 ml - -
HEPES 2.38 g 2.38 9.987
Distilled water 1000.00 ml - -
1 After sterilization add from sterile stock solutions add:
MgSO4 x 7 H2O
(1M) 		2.00 ml/l 0.493 2
Sodium acetate
(2M) 		10.00 ml/l 1.641 20
Yeast extract
(10%) 		2.00 ml/l 0.2 -
2 Adjust pH to 7.5. Dispense the medium into sealed bottles and autoclave.

Trace elements solution (Pfennig, 1965) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2
Halovibrio variabilis DSM 3050

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 120 2053.39
K2HPO4 3 17.224
HEPES 2.38 9.987
Sodium acetate 1.641 20
NH4Cl 0.5 9.348
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 2 strains
phyla 1 3 strains
oxygen facultative anaerobe 2 strains
temperature mesophilic 2 strains