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DSMZ

DSMZ 1166 . DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Desulfitispora elongata DSM 29990

* Modification: Replace formate and pyruvate with 20.00 ml/l of a 1 M Na-crotonate solution sterilized by filtration and added after autoclaving (see medium 870).

Growth conditions: 3-7 days anaerobic 35 °C

Main sol. 1166 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 6.00 g 5.982 102.362
K2HPO4 1.00 g 0.997 5.724
Na2SO4 2.80 g 2.792 19.654
NaHCO3 8.00 g 7.976 94.945
Na2CO3 22.00 g 21.934 206.95
NH4Cl 0.20 g 0.199 3.728
MgCl2 x 6 H2O 0.20 g 0.199 0.981
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 0.05 g 0.05 -
Na-formate* 3.40 g 3.39 49.845
Na-pyruvate* 0.55 g 0.548 4.983
Na2S2O3 x 5 H2O 5.00 g 4.985 20.086
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.24 g 0.239 0.996
Na-crotonate solution (1 M)* 20.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, hydrogenphosphate and sodium sulfate, then sparge solution with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, formate, pyruvate, thiosulfate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of vitamins and thiosulfate should be sterilized by filtration. Adjust pH of complete medium to 9.5 - 10.

Trace elements solution (Pfennig & Lippert,1966) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Na-crotonate solution (1 M) * #

1000
Solution occurs in 3 other media (first defined in Medium 870).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Crotonic acid
(ALDRICH 113018) 		86 g 86 998.955
NaOH
(10 N) 		100 ml - -
Distilled water 900 ml - -
1 Dissolve crotonic acid in 800 ml distilled water, add around 100 ml of 10 N NaOH and adjust pH to around 7. Then add water to reach a volume of 1000 ml. Sterilize by filtration under 100% N2 gas atmosphere.

Metadata

Last modified: 16.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5 - 10.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 17
Strains with modifications: 12
Desulfitispora elongata DSM 29990

  Na-formate
  Na-pyruvate
+  20 ml Na-crotonate solution (1 M)

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2CO3 21.934 206.95
NaHCO3 7.976 94.945
NaCl 5.982 102.362
Na2S2O3 x 5 H2O 4.985 20.086
Na2SO4 2.792 19.654
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Cultivation metadata from 17 strains BacDive

pH 8 - 10 3 strains
pH 10 - 12 3 strains
phyla 4 17 strains
oxygen anaerobe 13 strains
oxygen obligate anaerobe 1 strain
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