1107 .
DIALISTER MEDIUM
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Equipment needed:
Filter
Final gas composition:
10 % CO2
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Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
DIALISTER MEDIUM | 10.00 | ml | ||
| 1 To 10 ml of the dialister medium add aseptically: | |||||
|
|
Na-L-lactate
(50% (w/v)) |
0.10 | ml | ||
|
|
Haemin
(0.5 mg/ml) |
0.10 | ml | ||
|
|
NaHCO3
(10% (w/v)) |
0.10 | ml | ||
|
|
Glucose
(40% (w/v)) |
0.10 | ml | ||
|
|
Cystein-HCl x 2 H2O
(5% (w/v)) |
0.05 | ml | ||
|
|
DTT
(100 mM) |
0.05 | ml | ||
|
|
Vitamin K1 solution | 0.05 | ml | ||
|
|
Na-formate
(6% (w/v)) |
0.50 | ml | ||
|
|
Na-fumarate
(6% (w/v)) |
0.50 | ml | ||
|
|
Horse blood
(sterile) |
0.50 | ml | ||
| 2 Filter-sterilize all solutions and incubate them in oxygen-free atmosphere. | |||||
DIALISTER MEDIUM #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase
(BBL) |
17 | g | ||
|
|
Yeast extract | 3 | g | ||
|
|
NaCl | 3 | g | ||
|
|
KNO3 | 3 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
| 2 Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. | |||||
Vitamin K1 solution #
20
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin K1 | 0.1 | ml | ||
|
|
Ethanol
(95 %) |
20.0 | ml | ||
| 1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. | |||||