# 1107: DIALISTER MEDIUM


## Main sol. 1107

10	ml	**DIALISTER MEDIUM**	

1. To 10 ml of the dialister medium add aseptically:

0.1	ml	Na-L-lactate (50% (w/v))	
0.1	ml	Haemin (0.5 mg/ml)	
0.1	ml	NaHCO3 (10% (w/v))	
0.1	ml	Glucose (40% (w/v))	
0.05	ml	Cystein-HCl x 2 H2O (5% (w/v))	
0.05	ml	DTT (100 mM)	
0.05	ml	**Vitamin K1 solution**	
0.5	ml	Na-formate (6% (w/v))	
0.5	ml	Na-fumarate (6% (w/v))	
0.5	ml	Horse blood (sterile)	

2. Filter-sterilize all solutions and incubate them in oxygen-free atmosphere.


## DIALISTER MEDIUM

17	g	Trypticase (BBL)	
3	g	Yeast extract	
3	g	NaCl	
3	g	KNO3	
1000	ml	Distilled water	

1. Adjust pH to 7.0.
2. Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere.


## Vitamin K1 solution

0.1	ml	Vitamin K1	
20	ml	Ethanol (95 %)	

1. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.