1014
1014 .
THIOALKALIVIBRIO HALOPHILUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Thioalkalivibrio halophilus DSM 15791
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
30 °C
Main sol. 1014 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 175.00 | g | 174.825 | 2991.53 | |
| K2HPO4 | 1.50 | g | 1.499 | 8.603 | |
| NH4Cl | 0.50 | g | 0.5 | 9.338 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.983 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.013 | |
| Na2S2O3 | 5.00 | g | 4.995 | 11.657 | |
| NaHCO3 | 4.00 | g | 3.996 | 47.568 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve sodium chloride, hydrogenphosphate, ammonium chloride and trace elements and fill solution in screw capped Erlenmeyer flasks to 10% of volume. Autoclave at 110°C for 30 min. Add magnesium chloride, magnesium sulfate, thiosulfate and bicarbonat from sterile stock solutions and adjust pH of the medium to 8.0 - 8.5. | |||||
Solution occurs in 28
other media (first defined in Medium 1369).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.03 | g | 0.03 | 0.485 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 3.0-4.0 | |||||
Metadata
| Last modified: | 12.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 8.0 - 8.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thioalkalivibrio halophilus DSM 15791
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 174.825 | 2991.53 |
| Na2S2O3 | 4.995 | 11.657 |
| NaHCO3 | 3.996 | 47.568 |
| K2HPO4 | 1.499 | 8.603 |
| NH4Cl | 0.5 | 9.338 |
| Show more | ||
