1014 .
THIOALKALIVIBRIO HALOPHILUS MEDIUM
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Selected strain: Thioalkalivibrio halophilus DSM 15791
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
30 °C
Equipment needed:
Autoclave
Final pH:
8.0 - 8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 175.00 | g | ||
|
|
K2HPO4 | 1.50 | g | ||
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NH4Cl | 0.50 | g | ||
|
|
Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | ||
|
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MgCl2 x 6 H2O | 0.20 | g | ||
|
|
MgSO4 x 7 H2O | 0.25 | g | ||
|
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Na2S2O3 | 5.00 | g | ||
|
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NaHCO3 | 4.00 | g | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve sodium chloride, hydrogenphosphate, ammonium chloride and trace elements and fill solution in screw capped Erlenmeyer flasks to 10% of volume. Autoclave at 110°C for 30 min. Add magnesium chloride, magnesium sulfate, thiosulfate and bicarbonat from sterile stock solutions and adjust pH of the medium to 8.0 - 8.5. | |||||
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
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FeSO4 x 7 H2O | 2.20 | g | ||
|
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ZnSO4 x 7 H2O | 0.10 | g | ||
|
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MnCl2 x 4 H2O | 0.03 | g | ||
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H3BO3 | 0.03 | g | ||
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CoCl2 x 6 H2O | 0.20 | g | ||
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CuCl2 x 2 H2O | 0.03 | g | ||
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NiCl2 x 6 H2O | 0.03 | g | ||
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Na2MoO4 x 2 H2O | 0.03 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||