Main sol. 483
Internal Solution-ID: S985
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.297 | 2.183 | |
| K2HPO4 | 0.3 | g | 0.297 | 1.705 | |
| NH4Cl | 1.0 | g | 0.99 | 18.51 | |
| NaCl | 1.0 | g | 0.99 | 16.942 | |
| MgCl2 x 6 H2O | 1.0 | g | 0.99 | 4.87 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.099 | 0.673 | |
| KCl | 0.1 | g | 0.099 | 1.328 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| Yeast extract | 2.0 | g | 1.98 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.495 | 2.819 | |
| Na2CO3 | 1.0 | g | 0.99 | 9.342 | |
| D-Glucose | 4.0 | g | 3.96 | 21.983 | |
| Na2S x 9 H2O | 0.3 | g | 0.297 | 1.237 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine, carbonate, glucose and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 6.5, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add glucose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to pH 7.2 -7.4, if necessary. | |||||
Media with this solution
| 483 | DEFLUVIITALEA MEDIUM |