Main sol. 419
Internal Solution-ID: S850
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.25 | g | 0.25 | 1.432 | |
| KH2PO4 | 0.25 | g | 0.25 | 1.833 | |
| (NH4)2SO4 | 0.50 | g | 0.499 | 3.776 | |
| MgSO4 x 7 H2O | 25.00 | mg | 0.025 | 0.101 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na-oxalate | 5.00 | g | 4.99 | 37.239 | |
| Na-acetate | 0.82 | g | 0.818 | 9.976 | |
|
Yeast extract
(OXOID) |
1.00 | g | 0.998 | - | |
|
Trypticase peptone
(BD BBL) |
1.00 | g | 0.998 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 4.00 | g | 3.992 | 37.665 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.499 | 2.841 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate and cysteine, adjust pH to 6.8, bring medium to the boil and then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate and cysteine, then equilibrate pH to 6.8. Dispense under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Media with this solution
| 419 | OXALOBACTER MEDIUM |