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Main sol. 388

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Internal Solution-ID: S787

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.50 g 1.481 10.881
Na2HPO4 x 12 H2O 4.20 g 4.146 29.206
NH4Cl 0.50 g 0.494 9.228
MgCl2 x 6 H2O 0.38 g 0.375 1.845
CaCl2 x 2 H2O 0.06 g 0.059 0.403
Fe(NH4)2(SO4)2 x 6 H2O 0.04 g 0.039 -
CoCl2 x 6 H2O
(0.1% w/v) 		2.90 ml 0.003 0.022
Na2MoO4 x 2 H2O
(0.1% w/v) 		2.40 ml 0.002 0.01
Na2SeO3 x 5 H2O
(0.01% w/v) 		1.70 ml 1.7e-4 6.4e-4
MnCl2 x 4 H2O
(0.1% w/v) 		2.00 ml 0.002 0.01
ZnSO4
(0.1% w/v) 		2.80 ml 0.003 0.017
Yeast extract 2.00 g 1.974 -
Polypeptone 2.00 g 1.974 -
Starch
(soluble) 		5.00 g 4.936 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
Na2CO3 1.00 g 0.987 9.314
L-Cysteine HCl x H2O 1.00 g 0.987 5.621
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, cysteine and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary.
388 DICTYOGLOMUS MEDIUM