Main sol. 479b
Internal Solution-ID: S6395
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 50.00 | g | 49.505 | 847.107 | |
| KCl | 1.50 | g | 1.485 | 19.922 | |
| MgCl2 x 6 H2O | 6.00 | g | 5.941 | 29.22 | |
| CaCl2 x 2 H2O | 0.40 | g | 0.396 | 2.694 | |
| NH4Cl | 1.00 | g | 0.99 | 18.51 | |
| K2HPO4 x 3 H2O | 0.40 | g | 0.396 | 2.274 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 1.98 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 1.98 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2S x 9 H2O | 0.25 | g | 0.248 | 1.031 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except sulfide. Sparge medium with 100% N2 gas atmosphere for 30 - 45 min to make it anoxic, then adjust pH to 7.5 - 8.0 with NaOH and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials. After autoclaving, add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. Adjust the pH of the complete medium to 8.0, if necessary. | |||||