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NaHCO3 buffer (1M)
Internal Solution-ID: S6282
This solution is used in 3 media and was first defined in medium 1630c.
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaHCO3 | 4.2 | g | 84 | 999.917 | |
| Double distilled water | 50.0 | ml | - | - | |
| 1 Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 µm) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 °C. Avoid high headspace to liquid ratios to ensure stable concentration. | |||||