MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1844 . Nitrosotalea (AOA) Fresh Water Medium (FW)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1844 #

ml
Equipment needed: Autoclave Filter
1 Crucial: prepare all solutions in either clean glassware (incubated in 0.1 % HCl and washed with double distilled water) or GREINER PS container (201172). Do not use container with rubber stoppers or septum.
Compound Amount Unit Conc. [g/L] Conc. [mM]
FW basal salt solution (10x) 1000.00 ml - -
NH4Cl (1 M) 0.50 ml - -
NaHCO3 buffer (1M) 2.00 ml - -
Trace element solution (Nitrososphaera) 1.00 ml - -
FeNaEDTA solution (7.5 mM) 1.00 ml - -
MES Hydrat 1.95 g - -
2 Add 100 mL of 10x FW basal salt solution to 800 mL sterile double distilled water just before use.
3 Add per litre of 1x FW basalt solution: 0.5 mL NH4Cl (1 M), 2 mL NaHCO3 (1 M), 1 mL FeNaEDTA (7.5 mM), 1 mL trace elements solution, 1.95 g MES Hydrat.
4 Adjust pH to the specific pH for each strain.
5 Make up to 1000 ml with double distilled water.
6 Filtrate with 0.2 µm Nalgene® bottle-top sterile filter units (e.g. ThermoFischer 597-4520) in a clean and sterile glass bottle.
7 Aliquote medium in GREINER PS containers. Final medium can be stored at 4 °C in the dark for weeks.

FW basal salt solution (10x) #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 10 g 10 171.116
MgCl2 x 6 H2O 4 g 4 19.675
CaCl2 x 2 H2O 1 g 1 6.802
KH2PO4 2 g 2 14.697
KCl 5 g 5 67.069
1 Prepare FW basal salt solution (10x) in double distilled water.
2 Autoclave at 121 °C for 20 min.
3 Cool down to room temperature.
4 10x FW basal salt solution can be stored at 4 °C for months.

NH4Cl (1 M) #

50
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 2.67 g 53.4 998.317
Double distilled water 50.00 ml - -
1 Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water.
2 Filtrate (0.2 µm).

NaHCO3 buffer (1M) #

50
Solution occurs in 2 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 4.2 g 84 999.917
Double distilled water 50.0 ml - -
1 Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 µm) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 °C. Avoid high headspace to liquid ratios to ensure stable concentration.

Trace element solution (Nitrososphaera) #

1000
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(12.5M) 		8 mL 3.646 100
H3BO3 30 mg 0.03 0.485
MnCl2 x 4 H2O 100 mg 0.1 0.505
CoCl2 x 6 H2O 190 mg 0.19 1.463
NiCl2 x 6 H2O 24 mg 0.024 0.185
CuCl2 x 2 H2O 2 mg 0.002 0.012
ZnSO4 x 7 H2O 144 mg 0.144 0.501
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Double distilled water 1000 ml - -
1 This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.

FeNaEDTA solution (7.5 mM) #

1000
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 2753 mg 2.753 -
Double distilled water 1000 ml - -
1 Sterilise by filtration (0.2 µm) and store in the dark at 4 °C.

Metadata

Last modified: 17.09.24
Source: MediaDive: DSMZ
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.1
Equipment needed: Autoclave, Filter
Description:

Medium for the cultivation of AOA originating from freshwater habitats. Especially, Nitrosotalea (N. devanaterra, N. sinensis).
Edit in Medium builder

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 10 171.116
KCl 5 67.069
MgCl2 x 6 H2O 4 19.675
KH2PO4 2 14.697
CaCl2 x 2 H2O 1 6.802
Show more
CSV
JSON