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Trace element solution (Nitrososphaera)

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Internal Solution-ID: S6280

This solution is used in 4 media and was first defined in medium 1630c.

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(12.5M) 		8 mL 3.646 100
H3BO3 30 mg 0.03 0.485
MnCl2 x 4 H2O 100 mg 0.1 0.505
CoCl2 x 6 H2O 190 mg 0.19 1.463
NiCl2 x 6 H2O 24 mg 0.024 0.185
CuCl2 x 2 H2O 2 mg 0.002 0.012
ZnSO4 x 7 H2O 144 mg 0.144 0.501
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Double distilled water 1000 ml - -
1 This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.
1630e Synthetic Crenarchaeota Medium (SCM)
1630c modified Nitrosospaera medium
1844 Nitrosotalea (AOA) Fresh Water Medium (FW)
1845 Nitrososphaera gargensis medium