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Trace metals stock solution (chelated)

Internal Solution-ID: S6111

This solution is used in 3 media and was first defined in medium C3.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 3.2400 g 3.24 9.637
FeCl3 x 6 H2O 1.0800 g 1.08 3.996
MnSO4 x 4 H2O 0.4500 g 0.45 2.017
ZnSO4 x 7 H2O 0.2300 g 0.23 0.8
Na2MoO4 x 2 H2O 0.0970 g 0.097 0.401
CuSO4 x 5 H2O 0.0100 g 0.01 0.04
CoSO4 x 7 H2O 0.0056 g 0.006 0.036
1 Make up to 1 litre with distilled water and adjust pH to 7.6 ­- 7.8 with dilute HCl or NaOH. Store frozen.
C3 ANT
C29 E26 + Biotin:ANT
C32 E31:ANT