Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KNO3 | 0.0500 | g | 0.05 | 0.495 | |
NaH2PO4 x 2 H2O | 0.0078 | g | 0.008 | 0.065 | |
Tris | 1.0000 | g | 1 | 8.255 | |
Glycine | 0.3000 | g | 0.3 | 3.996 | |
Trace metals stock solution (chelated) | 2.5000 | ml | - | - | |
Thiamine HCl | 500.0000 | μg | - | - | |
Cyanocobalamine | 2.0000 | μg | - | - | |
Biotine | 1.0000 | μg | - | - | |
Natural sea water
(filtered) |
800.0000 | ml | - | - | |
1 Make up to 1 litre with distilled water and autoclave at 15 psi. Final pH should be 7.6 - 7.8. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA x 2 H2O | 3.2400 | g | 3.24 | 9.637 | |
FeCl3 x 6 H2O | 1.0800 | g | 1.08 | 3.996 | |
MnSO4 x 4 H2O | 0.4500 | g | 0.45 | 2.017 | |
ZnSO4 x 7 H2O | 0.2300 | g | 0.23 | 0.8 | |
Na2MoO4 x 2 H2O | 0.0970 | g | 0.097 | 0.401 | |
CuSO4 x 5 H2O | 0.0100 | g | 0.01 | 0.04 | |
CoSO4 x 7 H2O | 0.0056 | g | 0.006 | 0.036 | |
1 Make up to 1 litre with distilled water and adjust pH to 7.6 - 7.8 with dilute HCl or NaOH. Store frozen. |
Last modified: | 07.10.22 |
Source: | CCAP |
Synonyms: | Antia's Medium |
Medium type: | Defined medium |
Final pH: | 7.6 - 7.8 |
Description:
Referece: Antia NJ & Kalmakoff J (1965) Fish. Res. Bd Can., Manuscr. Rep. Ser. (Oceanogr. Limnol.) No. 203 - Antia NJ, Cheng JY & Taylor FJR (1969) Proc. Int. Seaweed Symp. 6, 1729 |
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