Main sol. 333
Internal Solution-ID: S611
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.4 | g | 0.4 | 7.478 | |
| MgSO4 x 7 H2O | 0.8 | g | 0.8 | 3.246 | |
| Trace element solution | 10.0 | ml | - | - | |
| KH2PO4 | 4.0 | g | 4 | 29.393 | |
| K2HPO4 | 4.0 | g | 4 | 22.965 | |
| K2S4O6 | 3.0 | g | 3 | - | |
|
Bromocresol purple
(saturated aq. solution) |
2.0 | ml | - | - | |
|
Agar
, for solid medium
(BD Bacto) |
10.0 | g | 10 | 29.733 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except phosphates and tetrathionate. The phosphates are sterilized separately in 1/10 of medium volume and mixed with the other salts when cool. Tetrathionate is sterilized by filtration and added to the autoclaved medium. Adjust pH of complete medium to 6.9. For agar medium autoclave the phosphates (in 100 ml), other salts (in 300 ml), and agar (in 600 ml) separately. | |||||
Media with this solution
| 333 | THERMITHIOBACILLUS MEDIUM |