Main sol. 1690
Internal Solution-ID: S5789
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| KCl | 1.50 | g | 1.5 | 20.121 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| NaCl | 11.68 | g | 11.68 | 199.863 | |
|
Distilled water
(MilliQ) |
1000.00 | ml | - | - | |
| CaCO3 | 4.00 | g | 4 | 39.964 | |
| 1 Dilute stock solution with MilliQ water (1+19 v/v), add 4 g CaCO3 per litre and autoclave. After autoclaving add sterile solutions: | |||||
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Borate-copper solution | 1.00 | ml | - | - | |
| Ammonium chloride solution | 10.00 | ml | - | - | |
| 2 Complete medium can be stored at 4°C. | |||||
| 3 Check pH 7.6-8.0 | |||||
Media with this solution
| 1690 | NITROSPIRA INOPINATA MEDIUM |