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DSMZ

DSMZ 1690 . NITROSPIRA INOPINATA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1690 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FWM stock solution 50 ml - -
Distilled water
(MilliQ) 		1000 ml - -
CaCO3 4 g 4 39.964
1 Dilute stock solution with MilliQ water (1+19 v/v), add 4 g CaCO3 per litre.
2 Autoclave at 120 °C for 21 min.
3 Cool down to room temperature.
4 After autoclaving add sterile solutions:
Trace element solution SL-10 1 ml - -
Selenite-tungstate solution 1 ml - -
Borate-copper solution 1 ml - -
Ammonium chloride solution 10 ml - -
5 Check pH 7.6-8.0
6 Complete medium can be stored at 4°C.
7 Incubate at 42°C without shaking. Activity and growth can be followed by measuring the depletion of ammonium or the production of nitrite and/or nitrate. Higher ammonium concentrations are inhibitive. For that reason our recommendation is follow nitrite content. As soon as nitrite has been produced and is depleting again (to form nitrate) another dose of ammonium can be added. Incubation time may be up to several weeks. We checked the nitrite content (absence/presence) every 5-7 days and added ammonium chloride about every 2-3 weeks. Don't expose cultures to intense light.

Borate-copper solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 54 mg 0.054 0.873
CuCl2 x 2 H2O 18 mg 0.018 0.106
Distilled water 1000 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 267 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 180 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Ammonium chloride solution #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ammonium chloride 0.8 g 8 149.561
Distilled water 100.0 ml - -

FWM stock solution #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g/l 1 7.348
KCl 1.50 g/l 1.5 20.121
MgSO4 x 7 H2O 1.00 g/l 1 4.057
NaCl 11.68 g/l 11.68 199.863
1 Make up to 1000 ml with MQ water.
2 Autoclave at 120 °C for 21 min.

Metadata

Last modified: 12.12.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.6 - 8.0
Equipment needed: Autoclave
Reference: doi.org/10.1038/nature23679
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
CaCO3 4 39.964
NaCl 0.584 9.993
Ammonium chloride 0.08 1.496
KCl 0.075 1.006
KH2PO4 0.05 0.367
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain
temperature thermophilic 1 strain