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Main sol. 1107

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Internal Solution-ID: S5719

Compound Amount Unit Conc. [g/L] Conc. [mM]
DIALISTER MEDIUM 10.00 ml - -
1 To 10 ml of the dialister medium add aseptically:
Na-L-lactate
(50% (w/v)) 		0.10 ml 0.05 -
Haemin
(0.5 mg/ml) 		0.10 ml - -
NaHCO3
(10% (w/v)) 		0.10 ml 0.01 0.119
Glucose
(40% (w/v)) 		0.10 ml 0.04 0.222
Cystein-HCl x 2 H2O
(5% (w/v)) 		0.05 ml 0.003 -
DTT
(100 mM) 		0.05 ml 7.7e-4 0.005
Vitamin K1 solution 0.05 ml - -
Na-formate
(6% (w/v)) 		0.50 ml 0.03 0.441
Na-fumarate
(6% (w/v)) 		0.50 ml 0.03 0.187
Horse blood
(sterile) 		0.50 ml - -
2 Filter-sterilize all solutions and incubate them in oxygen-free atmosphere.
1107 DIALISTER MEDIUM