This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
DIALISTER MEDIUM | 10.00 | ml | - | - | |
1 To 10 ml of the dialister medium add aseptically: | |||||
Na-L-lactate
(50% (w/v)) |
0.10 | ml | 0.05 | - | |
Haemin
(0.5 mg/ml) |
0.10 | ml | - | - | |
NaHCO3
(10% (w/v)) |
0.10 | ml | 0.01 | 0.119 | |
Glucose
(40% (w/v)) |
0.10 | ml | 0.04 | 0.222 | |
Cystein-HCl x 2 H2O
(5% (w/v)) |
0.05 | ml | 0.003 | - | |
DTT
(100 mM) |
0.05 | ml | 7.7e-4 | 0.005 | |
Vitamin K1 solution | 0.05 | ml | - | - | |
Na-formate
(6% (w/v)) |
0.50 | ml | 0.03 | 0.441 | |
Na-fumarate
(6% (w/v)) |
0.50 | ml | 0.03 | 0.187 | |
Horse blood
(sterile) |
0.50 | ml | - | - | |
2 Filter-sterilize all solutions and incubate them in oxygen-free atmosphere. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase
(BBL) |
17 | g | 17 | - | |
Yeast extract | 3 | g | 3 | - | |
NaCl | 3 | g | 3 | 51.335 | |
KNO3 | 3 | g | 3 | 29.673 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH to 7.0. | |||||
2 Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin K1 | 0.1 | ml | - | - | |
Ethanol
(95 %) |
20.0 | ml | 19 | 412.416 | |
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Filter |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Trypticase | 14.167 | - |
NaCl | 2.5 | 42.779 |
KNO3 | 2.5 | 24.727 |
Yeast extract | 2.5 | - |
Ethanol | 0.079 | 1.718 |
Show more |