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Main sol. 118

Internal Solution-ID: S5575

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Diaminopimelic acid 100 mg 0.1 0.526
Agar , if necessary 15 g 15 44.599
Distilled water 1000 ml - -
1 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
118 DAP-NUTRIENT AGAR