Main sol. J1014
Internal Solution-ID: S5057
Equipment needed: Filter
Autoclave
Anaerobic workbench
| 1 Mix components thoroughly and adjust pH to 6.0. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.20 | g | 0.194 | 1.468 | |
| KH2PO4 | 3.00 | g | 2.91 | 21.382 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.485 | 1.968 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.242 | 1.649 | |
| Yeast extract | 0.50 | g | 0.485 | - | |
| Modified Allen's trace metal solution | 1.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
| Iron(III) citrate solution | 100.00 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
|
L-Cysteine HCl
(25 mM) |
10.00 | ml | - | - | |
|
FeCl2
(130 mM) |
10.00 | ml | - | - | |
Media with this solution
| J1014 | NASU IRON REDUCER MEDIUM |