Solution A
Internal Solution-ID: S4811
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.000 | g | 27.655 | 473.218 | |
| KH2PO4 | 0.100 | g | 0.111 | 0.813 | |
| NH4Cl | 0.535 | g | 0.592 | 11.064 | |
| NaHCO3 | 2.000 | g | 2.212 | 26.336 | |
| Yeast extract | 1.000 | g | 1.106 | - | |
|
Tryptone
(BD-Difco) |
1.000 | g | 1.106 | 15.563 | |
| Glucose | 1.800 | g | 1.991 | 11.052 | |
| Sodium pyruvate | 0.550 | g | 0.608 | 5.529 | |
| Trace vitamins solution | 2.000 | ml | - | - | |
| Trace element solution | 1.000 | ml | - | - | |
| Se/W solution | 1.000 | ml | - | - | |
| Resazurin | 1.000 | mg | 0.001 | 0.005 | |
| Distilled water | 900.000 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Autoclave under a N2-CO2 (80:20, v/v) gas mixture and stand for overnight. | |||||
Media with this solution
| J846 | MO-CFXI MEDIUM |