Main sol. J841
Internal Solution-ID: S4805
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.198 | 1.458 | |
| NH4Cl | 0.25 | g | 0.248 | 4.637 | |
| NaCl | 1.00 | g | 0.992 | 16.976 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.397 | 1.952 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.149 | 1.012 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
|
Yeast extract
(BD-Difco) |
1.00 | g | 0.992 | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
Fructose
(10%) |
50.00 | ml | - | - | |
|
NaHCO3
(*, 5%) |
50.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - | |
| 2 Adjust pH to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| J841 | PROPIONISPORA MEDIUM |