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Main sol. 211

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Internal Solution-ID: S444

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.295 2.168
Na2HPO4 x 12 H2O 5.3 g 5.211 36.711
NH4Cl 1.0 g 0.983 18.383
MgCl2 x 6 H2O 0.2 g 0.197 0.967
Trace element solution 10.0 ml - -
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		1.5 ml 0.001 0.005
Yeast extract 1.0 g 0.983 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 4.9e-4 -
D-Glucose 5.0 g 4.916 27.289
Wolin's vitamin solution 5.0 ml - -
Na2S x 9 H2O 0.5 g 0.492 2.047
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except glucose, vitamins and sulfide), adjust pH to 6.0, sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into Hungate-type tubes or serum vials and autoclave. After sterilization add glucose and sulfide from anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized anoxic stock solution prepared under 100% N2. Adjust pH of complete medium to 6.0 - 6.5, if necessary.
211 THERMOANAEROBACTERIUM MEDIUM