Main sol. J341
Internal Solution-ID: S4060
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast cell paste | 5.0 | g | - | - | |
| Vitamin B12 | 0.5 | mg | 5.0e-4 | 3.7e-4 | |
| Agar | 15.0 | g | 15 | 44.599 | |
|
Sea water
(artificial) |
1000.0 | ml | - | - | |
| 1 Adjust pH to 7.5. Autoclave at 120C for 20 min. Separately sterilize vitamin B12 by filtration, and aseptically add to the medium. | |||||
Media with this solution
| J341 | VY/2-ASW AGAR |