Main sol. J176
Internal Solution-ID: S3834
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 3.00 | g | 3 | 22.703 | |
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| Ca(NO3)2 | 0.01 | g | 0.01 | 0.061 | |
|
Yeast extract
(BD-Difco) |
0.20 | g | 0.2 | - | |
|
Sulfur
(powder) |
10.00 | g | 10 | 311.818 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 2.0 with 10 N H2SO4. Autoclave yeast extract separately. Steam sulfur for 3 hr on each of 3 successive days. | |||||
Media with this solution
| J176 | ACIDIANUS BRIERLEYI MEDIUM |