Main sol. J174
Internal Solution-ID: S3832
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Brock's salt base solution | 1000.0 | ml | - | - | |
|
Yeast extract
(BD-Difco) |
0.5 | g | 0.5 | - | |
|
Sulfur
(powder) |
1.0 | g | 1 | 31.182 | |
| 1 Adjust pH to 2.5 with 10 N H2SO4. Autoclave yeast extract separately and steam sulfur for 3 hr on each of 3 successive days. Cultivate under air enriched with 1-10% CO2. | |||||
Media with this solution
| J174 | ACIDIANUS INFERNUS MEDIUM (A) |