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Main sol. 1571

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Internal Solution-ID: S3257

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.41 g 0.41 3.013
Na2HPO4 x 2 H2O 0.53 g 0.53 3.733
NH4Cl 0.30 g 0.3 5.609
NaCl 0.30 g 0.3 5.133
MgCl2 x 6 H2O 0.10 g 0.1 0.492
CaCl2 x 2 H2O 0.01 g 0.01 0.068
Yeast extract 2.00 g 2 -
Na2S x 9 H2O 0.36 g 0.36 1.499
Resazurin 0.50 g 0.5 2.182
Trace element solution SL-10 1.00 ml - -
Distilled water 1000.00 ml - -
L-Cysteine 0.50 g 0.5 4.127
1 Dissolve in 1 liter, boil under N2, add 0.5 g of L-cysteine and adjust pH to 6.4 (should be pH 5.5 after autoclaving). Disperse in tubes and bottles (leave 50% of container volume as gas phase). Change to gas mixture N2/CO2; 80/20%) and autoclave for 20 minutes at 121°C.
2 To 100 ml sterile medium add aseptically
Vitamin solution 0.50 ml - -
Selenite-tungstate solution 0.50 ml - -
1571 MICROBACTER MEDIUM