Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.41 | g | 0.41 | 3.013 | |
| Na2HPO4 x 2 H2O | 0.53 | g | 0.53 | 3.733 | |
| NH4Cl | 0.30 | g | 0.3 | 5.609 | |
| NaCl | 0.30 | g | 0.3 | 5.133 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Yeast extract | 2.00 | g | 2 | - | |
| Na2S x 9 H2O | 0.36 | g | 0.36 | 1.499 | |
| Resazurin | 0.50 | g | 0.5 | 2.182 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| L-Cysteine | 0.50 | g | 0.5 | 4.127 | |
| 1 Dissolve in 1 liter, boil under N2, add 0.5 g of L-cysteine and adjust pH to 6.4 (should be pH 5.5 after autoclaving). Disperse in tubes and bottles (leave 50% of container volume as gas phase). Change to gas mixture N2/CO2; 80/20%) and autoclave for 20 minutes at 121°C. | |||||
| 2 To 100 ml sterile medium add aseptically | |||||
| Vitamin solution | 0.50 | ml | - | - | |
| Selenite-tungstate solution | 0.50 | ml | - | - | |
Media with this solution
| 1571 | MICROBACTER MEDIUM |