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Mineral solution

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Internal Solution-ID: S3048

Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.7000 g 3.7 11.005
FeSO4 x 7 H2O 1.1000 g 1.1 3.957
ZnSO4 x 7 H2O 0.0288 g 0.029 0.1
MnCl2 x 4 H2O 0.0984 g 0.098 0.497
H3BO3 0.0310 g 0.031 0.501
CoCl2 x 6 H2O 0.0238 g 0.024 0.183
CuCl2 x 2 H2O 0.0172 g 0.017 0.101
Na2MoO4 x 2 H2O 0.0242 g 0.024 0.1
Distilled water 1000.0000 ml - -
1 Adjust pH to 6.0 with KOH
1486 GBS salts modified for Thermocrinis jamiesonii