1486
1486 .
GBS salts modified for Thermocrinis jamiesonii
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1486 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaH2PO4 | 1.380 | g | 1.38 | 11.502 | |
| NaCl | 3.000 | g | 3 | 51.335 | |
| NH4Cl | 0.107 | g | 0.107 | 2 | |
| CaCl2 x 2 H2O | 0.015 | g | 0.015 | 0.102 | |
| MgSO4 x 7 H2O | 0.123 | g | 0.123 | 0.499 | |
| KCl | 0.150 | g | 0.15 | 2.012 | |
| Na2SO4 | 0.300 | g | 0.3 | 2.112 | |
| Mineral solution | 5.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 Prepare the medium under a nitrogen atmosphere. Dispense 5 ml into 25 ml tubes fitted with rubber stoppers. | |||||
| 3 Autoclave at 121 °C for 15 min. | |||||
| 4 after autoklaving, add the following from anaerobic, sterile (autoclaved) stock solutions (e.g., 100x) | |||||
| Sodium acetate trihydrate | 2.000 | mM | 0.272 | 2 | |
| Sodium thiosulfate pentahydrate | 2.000 | mM | 0.496 | 2 | |
| Sodium bicarbonate | 1.000 | mM | - | 1 | |
| 5 Before inoculation 2% sterile O2 or 10% sterile air is added to the headspace. | |||||
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 3.7000 | g | 3.7 | 11.005 | |
| FeSO4 x 7 H2O | 1.1000 | g | 1.1 | 3.957 | |
| ZnSO4 x 7 H2O | 0.0288 | g | 0.029 | 0.1 | |
| MnCl2 x 4 H2O | 0.0984 | g | 0.098 | 0.497 | |
| H3BO3 | 0.0310 | g | 0.031 | 0.501 | |
| CoCl2 x 6 H2O | 0.0238 | g | 0.024 | 0.183 | |
| CuCl2 x 2 H2O | 0.0172 | g | 0.017 | 0.101 | |
| Na2MoO4 x 2 H2O | 0.0242 | g | 0.024 | 0.1 | |
| Distilled water | 1000.0000 | ml | - | - | |
| 1 Adjust pH to 6.0 with KOH | |||||
Metadata
| Last modified: | 26.10.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.2 - 7.3 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 3 | 51.335 |
| NaH2PO4 | 1.38 | 11.502 |
| Sodium thiosulfate pentahydrate | 0.496 | 2 |
| Na2SO4 | 0.3 | 2.112 |
| Sodium acetate trihydrate | 0.272 | 2 |
| Show more | ||
