Main sol. 1271
Internal Solution-ID: S2537
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.5 | g | 0.499 | 9.32 | |
| KH2PO4 | 0.1 | g | 0.1 | 0.733 | |
| NaCl | 25.0 | g | 24.925 | 426.51 | |
| MgCl2 x 6 H2O | 4.0 | g | 3.988 | 19.616 | |
| CaCl2 x 2 H2O | 1.0 | g | 0.997 | 6.782 | |
| Yeast extract | 1.0 | g | 0.997 | - | |
| D-Glucose | 1.7 | g | 1.695 | 9.408 | |
| Trace element solution | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.5 | g | 1.496 | 14.11 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.299 | 1.703 | |
| Na2S x 9 H2O | 0.3 | g | 0.299 | 1.245 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, vitamins, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.3 - 7.5, if necessary. | |||||
Media with this solution
| 1271 | PLEOMORPHOCHAETA MEDIUM |