Main sol. 1249
Internal Solution-ID: S2481
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 17.00 | g | 16.966 | 290.316 | |
| NaNO3 | 2.55 | g | 2.545 | 29.942 | |
| MgCl2 x 6 H2O | 4.50 | g | 4.491 | 22.09 | |
| CaCl2 x 2 H2O | 0.35 | g | 0.349 | 2.376 | |
| NH4Cl | 1.00 | g | 0.998 | 18.658 | |
| KH2PO4 | 0.08 | g | 0.08 | 0.587 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na2CO3 | 1.50 | g | 1.497 | 14.124 | |
| Na-acetate | 1.00 | g | 0.998 | 12.166 | |
| Yeast extract | 3.00 | g | 2.994 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, acetate and yeast extract), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add acetate and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be at 7.0 - 7.2. | |||||
Media with this solution
| 1249 | GEOALKALIBACTER SUBTERRANEUS MEDIUM |