Main sol. 1076
Internal Solution-ID: S2171
This solution is used in 2 media and was first defined in medium 1076.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 5.00 | g | 9.709 | 136.589 | |
| Peptone | 2.65 | g | 5.146 | - | |
| PPLO broth | 1.75 | g | 3.398 | - | |
| Distilled water | 375.00 | ml | - | - | |
| 1 Adjust pH to 7.4; autoclave at 121°C for 15 minutes. | |||||
| 2 Add aseptically: | |||||
|
Fetal bovine serum
(heat-inactivated) |
90.00 | ml | - | - | |
|
CMRL 1066
(10x concentrated; GIBCO) |
25.00 | ml | - | - | |
|
Yeast extract
(20%, autoclaved) |
17.50 | ml | 3.5 | - | |
|
Yeast extract
(20%, autoclaved) |
5.00 | ml | 1 | - | |
|
Phenol red
(1%, filter-sterilized) |
1.00 | ml | 0.01 | 0.028 | |
|
L-Glutamine
(200 mM; GIBCO) |
1.70 | ml | 0.05 | 0.34 | |