Main sol. 998
Internal Solution-ID: S2035
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.3 | 2.205 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| MgCl2 x 6 H2O | 1.00 | g | 1 | 4.919 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| NaCl | 20.00 | g | 20 | 342.231 | |
| Trace element solution SL12 | 1.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Vitamins V7 solution (Pfennig et Trüper, 1992) | 1.00 | ml | - | - | |
| NaHCO3 | 0.15 | %(w/v) | 1.5 | 17.856 | |
| 1 Media were autoclaved and cooled under N2/CO2 (90/10, v/v). Vitamin V7 solution (1 ml/l) and NaHCO3, (0.15% w/v) were then aseptically added to the medium. Final pH was adjusted to 6.8-7.0 and the medium was dispensed into sterile 50 ml screw-capped bottles. Organic substrates (5 mM Na-acetate and/or 5 mM di-Na-succinate) were added as substrates before use. In addition, for strains DSM 15114 and AT2115, Na2S x 9 H2O (0.02% w/v) were also added to the medium prior to utilisation. Pure cultures, grown in 50-ml screw-capped bottles were stored at +4°C in the dark for preservation. | |||||
Media with this solution
| 998 | MEDIUM FOR ROSEOSPIRA |