Main sol. 782
Internal Solution-ID: S1587
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4-oxalate | 15.00 | g | 15 | 120.874 | |
| NaHCO3 | 10.00 | g | 10 | 119.038 | |
| NaH2PO4 x 2 H2O | 10.00 | g | 10 | 83.349 | |
| NaCl | 0.70 | g | 0.7 | 11.978 | |
| KCl | 0.57 | g | 0.57 | 7.646 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Na2S2O3 x 5 H2O | 1.00 | g | 1 | 4.029 | |
| Distilled water | 1000.00 | ml | - | - | |
|
Agar
, if required
(adjust if required in range 1.5-2.0) |
2.00 | %(w/v) | 20 | 59.465 | |
| 1 pH 6.8-7.0 | |||||
| 2 The first three salts must be dissolved in the indicated sequence. After autoclaving for 20 min at 121°C the pH of the medium shifted to 8.5-9.0. Solid medium contained 1.5 to 2.0% (w/v) of Bacto-Agar (Difco). | |||||
Media with this solution
| 782 | OM-2 MEDIUM |