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Main sol. 614

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Internal Solution-ID: S1320

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.326 4.378
MgCl2 x 6 H2O 0.52 g 0.514 2.53
CaCl2 x 2 H2O 0.22 g 0.218 1.48
NH4Cl 0.33 g 0.326 6.102
KH2PO4 0.33 g 0.326 2.399
Yeast extract 0.50 g 0.495 -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
NaHCO3 1.00 g 0.989 11.774
D-Fructose 10.00 g 9.891 54.902
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.70 g 0.692 2.883
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, vitamins, fructose and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add vitamins, fructose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 6.8 - 7.0, if necessary.
614 ACETOBACTERIUM FIMETARIUM MEDIUM