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Trace element solution

Internal Solution-ID: S1238

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.0 g 50 148.718
ZnSO4 x 7 H2O 2.2 g 2.2 7.651
CaCl2 x 2 H2O 5.5 g 5.5 37.411
MnCl2 x 4 H2O 5.1 g 5.1 25.77
FeSO4 x 7 H2O 5.0 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.1 g 1.1 -
CuSO4 x 5 H2O 1.6 g 1.6 6.408
CoCl2 x 6 H2O 1.6 g 1.6 12.323
Distilled water 1000.0 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7 using 2 N KOH. Adjust pH of final solution to 6.0 with KOH.
574 GUYPARKERIA HYDROTHERMALIS MEDIUM