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DSMZ

DSMZ 574 . GUYPARKERIA HYDROTHERMALIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 574 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.0 g 25 427.789
Na2S2O3 x 5 H2O 2.5 g 2.5 10.073
(NH4)2SO4 1.0 g 1 7.568
MgSO4 x 7 H2O 1.5 g 1.5 6.086
KH2PO4 0.4 g 0.4 2.939
NaHCO3 0.2 g 0.2 2.381
CaCl2 x 2 H2O 0.3 g 0.3 2.041
Phenol red
(0.5% w/v) 		2.0 ml 0.01 0.028
Trace element solution 1.0 ml - -
Tris-HCl buffer , 0.1 M, pH 7.5 200.0 ml - -
Distilled water 800.0 ml - -
1 Dissove ingredients and adjust pH to 7.5. Sterilize the medium by filtration.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.0 g 50 148.718
ZnSO4 x 7 H2O 2.2 g 2.2 7.651
CaCl2 x 2 H2O 5.5 g 5.5 37.411
MnCl2 x 4 H2O 5.1 g 5.1 25.77
FeSO4 x 7 H2O 5.0 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.1 g 1.1 -
CuSO4 x 5 H2O 1.6 g 1.6 6.408
CoCl2 x 6 H2O 1.6 g 1.6 12.323
Distilled water 1000.0 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7 using 2 N KOH. Adjust pH of final solution to 6.0 with KOH.

Metadata

Last modified: 05.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
Na2S2O3 x 5 H2O 2.5 10.073
MgSO4 x 7 H2O 1.5 6.086
(NH4)2SO4 1 7.568
KH2PO4 0.4 2.939
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Cultivation metadata from 1 strains BacDive

halophily halophilic 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
nutrition chemoautolithotroph 1 strain
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