Strain modifications for Medium 9
8 distinct modifications were found.
with cellulose pads
Preferentially with 7.5 g/l yeast and 8 g/l agar
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1118 as well as on medium 9-agar plates
Rehydration of ampoules: Add about 0.7 - 1.0 ml modified DSMZ medium 1118 (per L: Casitone (pancreatic digest of casein) 3.0 g; Calciumchloride dihydrate 0.7 g; Magnesiumsulphate heptahydrate 2.0 g) to the vial, mix and allow the suspension to rehydrate for 30 min at room temperature. Transfer 0.5 ml to an agar plate containing medium 9 or 222
With 2 g/l maltose and sterile pieces of cellulosic (filter) paper (3-4 pieces (ca. 1cm x 1cm) per plate). Inoculate the pieces of paper
With or without 2 g/l maltose
With sterile pieces of cellulosic (filter) paper (3-4 pieces (ca. 1cm x 1cm) per plate). Inoculate the pieces of paper