JCM 99 . ALKALINE XYLAN AGAR

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Equipment needed:

Final pH: 10.0

Select a strain (optional):

ml
Compound Amount Unit
Larchwood xylan
(Sigma)
10.0 g
Yeast extract 5.0 g
Polypeptone
(Nihon Pharm. Co.)
5.0 g
K2HPO4 1.0 g
MgSO4 x 7 H2O 0.2 g
Agar 15.0 g
Distilled water 1000.0 ml
1 After autoclaving, adjust pH to 10.0 with sterilized 10% Na2CO3 solution.