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DSMZ

JCM 89 . SM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Thiobacillus thioparus DSM 505

This strain uses the standard medium without modifications.

Main sol. J89 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.5 g 1.493 10.968
Na2HPO4 4.5 g 4.478 31.542
NH4Cl 0.3 g 0.299 5.581
MgSO4 x 7 H2O 0.1 g 0.1 0.404
Na2S2O3
(10%) 		100.0 ml - -
Trace metal solution 5.0 ml - -
Distilled water 900.0 ml - -
1 Adjust pH to 7.5.
2 Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium.

Trace metal solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 50.00 g 50 171.092
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 5.54 g 5.54 49.919
MnCl2 x 4 H2O 5.06 g 5.06 -
FeSO4 x 7 H2O 4.99 g 4.99 17.949
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 -
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Adjust pH to 6.0 with KOH.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 2
Thiobacillus thioparus DSM 505

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 4.478 31.542
KH2PO4 1.493 10.968
NH4Cl 0.299 5.581
EDTA 0.249 0.851
ZnSO4 x 7 H2O 0.109 0.381
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains