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Main sol. J89

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Internal Solution-ID: S3718

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.5 g 1.493 10.968
Na2HPO4 4.5 g 4.478 31.542
NH4Cl 0.3 g 0.299 5.581
MgSO4 x 7 H2O 0.1 g 0.1 0.404
Na2S2O3
(10%) 		100.0 ml - -
Trace metal solution 5.0 ml - -
Distilled water 900.0 ml - -
1 Adjust pH to 7.5.
2 Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium.
J89 SM MEDIUM