676 .
PYG MEDIUM (G)
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Selected strain: Bacteroides faecis DSM 24798
This strain uses the standard medium without modifications.
Equipment needed:
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone | 5.0 | g | ||
|
|
Peptone | 5.0 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
Beef extract | 5.0 | g | ||
|
|
Glucose | 5.0 | g | ||
|
|
K2HPO4 | 2.0 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Tween 80 | 1.0 | ml | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
Salt solution | 40.0 | ml | ||
|
|
Distilled water | 940.0 | ml | ||
|
|
Hemin solution | 10.0 | ml | ||
|
|
Hemin solution | 10.0 | ml | ||
| 1 Adjust pH to 7.2. | |||||
Salt solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CaCl2 x 2 H2O | 0.25 | g |
|
|
MgSO4 x 7 H2O | 0.50 | g |
|
|
K2HPO4 | 1.00 | g |
|
|
KH2PO4 | 1.00 | g |
|
|
NaHCO3 | 10.00 | g |
|
|
NaCl | 2.00 | g |
|
|
Distilled water | 1000.00 | ml |