# J676: PYG MEDIUM (G)


## Main sol. J676

5	g	Trypticase peptone	
5	g	Peptone	
10	g	Yeast extract	
5	g	Beef extract	
5	g	Glucose	
2	g	K2HPO4	
0.5	g	L-Cysteine HCl x H2O	
1	ml	Tween 80	
1	mg	Resazurin	
40	ml	**Salt solution**	
940	ml	Distilled water	
10	ml	**Hemin solution**	
10	ml	**Hemin solution**	

1. Adjust pH to 7.2.


## Salt solution

0.25	g	CaCl2 x 2 H2O	
0.5	g	MgSO4 x 7 H2O	
1	g	K2HPO4	
1	g	KH2PO4	
10	g	NaHCO3	
2	g	NaCl	
1000	ml	Distilled water	


## Hemin solution

1. Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.